Advanced Research Assistant

6 days left

Wellcome Trust Sanger Institute
GBP24589 - GBP30995 per annum + Excelent benefits
02 Aug 2017
30 Aug 2017
Contract Type
Full Time

Salary range: GBP24589 to GBP30995pa plus excellent benefits

We are seeking two highly motivated and flexible individuals to join the GE team as an Advanced Research Assistant. The post holder's main responsibilities will be execution of day-to-day workflows of projects for the generation of targeting vectors, CRISPR/Cas9 reagents and the generation and validation of quality assured targeted mouse ES cells. Key responsibilities include execution and timely delivery of these resources, accurate record keeping, project troubleshooting and an ability to work across multiple projects. In addition, the group also has a research and development (R&D) commitment to implement new approaches that facilitate the insertion of complex alleles directly into the mouse zygote.

Previous experience of mammalian cell culture, especially mouse ES cell (mESC) culture is a prerequisite for this role. The candidate would also be expected to be able to genotype the mESC clones generated in order to provide quality assured reagents for microinjection. The candidate should have a broad knowledge of molecular biology techniques such as nucleic acid extraction, construct generation (via conventional cloning, Gibson assembly and/or recombineering), PCR, restriction enzyme analysis and sequencing. An understanding of conventional gene targeting and the more recent CRISPR/Cas9 genome engineering techniques would be advantageous. The successful candidate must be able to work as part of a team and also have the ability to independently handle complex workflows from project start to delivery. Good communication/record keeping skills are a key part of the role.

Some weekend cell culture is anticipated in this role.

Essential Skills:

  • Experienced in mammalian cell culture, especially routine mouse ES cells culturing.
  • An understanding and practical experience in molecular biology strategies and techniques, including some of the following: molecular cloning, construct generation (via conventional cloning, Gibson assembly and recombineering), PCR, restriction enzyme analysis and sequencing.
  • Isolation and purification of nucleic acid, particularly RNA based methodologies for the generation of CRISPR reagents.
  • Practical experience and theoretical understanding of genotyping techniques, including qPCR, long range PCR and sequencing and data interpretation there of.
  • Capable of performing/tracking a variety of challenging projects simultaneously
  • Ability to work as part of a team and also independently once appropriate training and guidance has been provided.
  • Good communication skills to allow accurate information to be exchanged
  • Science degree in biological sciences or extensive, proven practical experience
  • Ability to schedule and deliver simultaneous projects within agreed deadlines
  • Good computer skills

Ideal Skills:

  • Experience of transfection, nucleofection and electroporation technologies would be advantageous
  • Practical experience in understanding gene structures on standard genome browsers
  • Practical experience and theoretical understanding of gene targeting strategies
  • Ability to test ,implement and troubleshoot new approaches and techniques

Other Information:

The Sanger Institute is a charitably funded research centre focused on understanding the role of genetics in health and disease. We use state of the art large-scale genomic approaches to drive world-leading projects to uncover the basis of genetic and infectious disease. Our goal is to provide results that can be translated into diagnostics, treatments or therapies that reduce global health burdens.

Wellcome Trust Sanger Institute welcomes applications from all candidates irrespective of age, disability, gender, gender identity, sexual orientation, race, religion or belief, or marital or civil partnership status.

Please include a covering letter and CV with your application.

Closing date for applications: 3rd September 2017